REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Human plasma, NaHep, pooled gender	BioIVT	Cat# HMN378062
Chemicals, peptides, and recombinant proteins
Lysyl Endopeptidase (Lys-C)	Wako Chemicals	Cat# NC9242798
Sequencing Grade Modified Trypsin	Promega	Cat# V5111
Acetonitrile (Optima™ LC/MS)	Fisher Scientific	Cat# A9554 (CS)
Methanol (Optima™ LC/MS)	Fisher Scientific	Cat# A454SK-4
Water (Optima™ LC/MS)	Fisher Scientific	Cat# W6-4
TCEP HCl (tris(2-carboxyethyl)phosphine hydrochloride) for biotechnology	VWR	Cat# VWRVK831-10G
2-Chloroacetamide, ≥98.0% (HPLC)	Sigma-Aldrich	Cat# 22790-250G-F
Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade	Thermo Scientific	Cat# 28901
Guanidine hydrochloride, for molecular biology, ≥99%	Sigma-Aldrich	Cat# G3272-500G
Invitrogen™ Tris (1 M), pH 8.0, RNase-free	Fisher Scientific	Cat# AM9856
Urea powder, BioReagent, for molecular biology, suitable for cell culture	Sigma-Aldrich	Cat# U5378-1KG
Pierce™ Formic Acid, LC-MS Grade	Thermo Scientific	Cat# PI28905
tert-Butyl methyl ether, 1 L	Sigma-Aldrich	Cat# 443808-1L
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail	Roche	Cat# 11836170001
Critical commercial assays
Quantitative Colorimetric Peptide Assay	Pierce	Cat# 23275
BCA Protein Assay Kit - Reducing Agent Compatible	Pierce	Cat# 23250
Other
Strata™-X 33 μm Polymeric Reversed Phase, 10 mg / well, 96-Well Plates	Phenomenex	Part# 8E-S100-AGB
Strata™-X 33 μm Polymeric Reversed Phase, 10 mg / 1 mL, Tubes , 100/Pk	Phenomenex	Part# 8B-S100-AAK
Strata™-X 33 μm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes , 100/Pk	Phenomenex	Part# 8B-S100-TAK
Strata® 96-Well Plate Manifold, Universal, w/ Vacuum Gauge	Phenomenex	Part # AH0-8950
Whatman® Panpeha™ pH indicator strips, pH range 0 to 14, 200/pk	Millipore Sigma	Cat# WHA10360005
PrecisionGlide™ Hypodermic Conventional Needles	BD	Cat# 14-826-10
Heratherm Compact Incubator	Lab Depot, Inc.	Cat# 50125590
Digital Dry Baths/Block Heater with open container style block filled with sand	Thermo Scientific	Cat# 88870001
3′ Purifier Logic+ Class II A2 Biological Safety Cabinet with 10" sash opening	Labconco Corporation	Cat# 302310001
Ducted Chemical Fume Hood with a Vertical Sash	Labconco Corporation	Cat# 110410000
NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer	Thermo Scientific	Cat# ND-ONE-W
Q700 Sonicator	QSonica	Cat# Q700-110
ELx808™ Absorbance Microplate Reader	BioTek	n/a