Detection for CIAV and ALV-J in the infected HD11 cells. The green and blue columns represent the CIAV and CIAV+ALV-J groups, respectively. (A) Comparison of ALV-J from infected HD11 cells at 8 dpi detected by Western blot using mAb JE9 against ALV-J Env and anti-GAPDH antibody. Lane 1 to 3: HD11 cells infected with CIAV, ALV-J, and CIAV+ALV-J at 8 dpi, respectively. Lane 4: Negative HD11 cells considered as control. (B) Comparison of genome DNA copy numbers of CIAV in the supernatants from HD11 cells infected with CIAV and CIAV+ALV-J at 8 dpi, respectively, determined by q-PCR. Abbreviations: ALV-J, J avian leukosis virus; CIAV, chicken infectious anemia virus.