CIAV viral cloacal shedding and tissue viral load in the infected chickens The green and blue columns represent the CIAV and CIAV+ALV-J groups, respectively. (A) Comparison of the positive rates of CIAV viral cloacal shedding in chickens infected with CIAV and CIAV+ALV-J at 7, 35, and 63 dpi. (B–G) Comparison of CIAV viral loads in organs from chickens infected with CIAV and CIAV+ALV-J at 7, 14, 21, 35, 49, and 63 dpi, respectively. The data were analyzed with a Student t test. A P value of <0.05 was considered significant. Abbreviations: ALV-J, J avian leukosis virus; CIAV, chicken infectious anemia virus; LOD, limit of detection.