Fig. 4.

Validation of DEPs by Western blotting (WB).A, IBRS-2 cells were infected with 0.5 MOI of SVV for 6 h. The expression of KIF11, TRIB1, ZNF384, UBC13, cystatin C, and IGFBP2 was determined by WB analysis. The relative fold change of the DEPs was determined by densitometric analysis after normalized to β-actin (WB ratio) and compared with the iTRAQ labeling ratio. B, PK-15 cells were infected with 0.5 MOI of SVV for 6 h. The expression of KIF11, MX1, IFIT1, GSDMA, MAP4K4, and DEPD5 was detected by WB. The WB ratio and iTRAQ labeling ratio were revealed and compared. DEP, differentially expressed protein; IBRS-2, Instituto Biologico-Rim Suino-2; MOI, multiplicity of infection; PK-15, porcine kidney-15 cells; SVV, Seneca Valley virus.