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. 2021 Sep 14;20:100147. doi: 10.1016/j.mcpro.2021.100147

Fig. 8.

Fig. 8

The components of RLR signaling pathway-mediated IFN-β promoter activation in IBRS-2 and PK-15 cells.A, IBRS-2 cells or PK-15 cells were cotransfected with the plasmids expressing porcine RIG-I, MDA5, MAVS, or TBK1 together with the IFN-β promoter–driven luciferase reporter plasmids and the internal control pRL-TK plasmids. Luciferase activity was measured and analyzed at 24 h post-transfection. B, IBRS-2 cells or PK-15 cells were cotransfected with the plasmids expressing porcine IRF3 or IRF7 together with the IFN-β promoter–driven luciferase reporter plasmids and pRL-TK plasmids. Luciferase activity was measured and analyzed at 24 h post-transfection. The expression of these components was determined by Western blotting analysis. IBRS-2, Instituto Biologico-Rim Suino-2; IFN-β, interferon beta; IRF3, IFN regulatory factor 3; IRF7, IFN regulatory factor 7; PK-15, porcine kidney-15 cells; RLR, RIG-I–like receptor.