Figure 6.

miR-100 targets FOXA1. (A) Reverse transcription-quantitative PCR analysis was performed to assess the effect of altering miR-100 expression on FOXA1 mRNA expression. (B) Western blot analysis was performed to assess the effect of altering miR-100 expression on FOXA1 protein expression. (C) The dual-luciferase reporter assay demonstrated that miR-100 targeted the 3′-untranslated region of FOXA1. **P<0.01, ***P<0.001 vs. the NC or miR-100 mimic groups. miR, microRNA; FOXA1, forkhead box A1; NC, negative control; WT, wild-type; MUT, mutant.