Table 3.
Cost for analysis taking into account laboratory bench costs as well as salaries.
| 1 Sample + nc | 7 Samples + nc | 15 Samples + nc | |||||||
|---|---|---|---|---|---|---|---|---|---|
| DNA preparation kit | Total costs | Number of samples | Cost per sample | ||||||
| Qiagen Allprep DNA/RNA mini kit | 4,756.0 | 50 | 95.1 | 190.2 | 761.0 | 1,521.9 | |||
| PCR reagents | |||||||||
| Mytaq PCR reagents (BRAF exon 15) | 40.0 | 8 | 5.0 | 10.0 | 40.0 | 80.0 | |||
| Hotstar PCR reagents (BRAF V600E) | 100.0 | 8 | 12.5 | 25.0 | 100.0 | 200.0 | |||
| Detection | |||||||||
| Qiaxcel capillary gel electrophoresis | 100.0 for up to 12 samples | 100.0 | 100.0 | 200.0 | |||||
| Total material costs PCR/Qiaxcel method only | 325.2 | 1,001.0 | 2,001.9 | ||||||
| BMA salary costs PCR/Qiaxcel method only | 400.0 per hour | 5 | 2,000.0 | 5 | 2,000.0 | 6 | 2,400.0 | ||
| Total costs PCR/Qiaxcel method only | 2,325.2 | 3,001.0 | 4,401.9 | ||||||
| Costs per sample PCR/Qiaxcel method only | 2,325.2 | 428.7 | 293.5 | ||||||
| ddPCR | |||||||||
| Mastermix | 4,605.0 | 420 | 11.0 | 88.0 | 88.0 | 176.0 | |||
| Primer probes | 4,000.0 | 200 | 20.0 | 160.0 | 160.0 | 320.0 | |||
| Additional equipment (for maximum of eight samples) | 300.0 | 300.0 | 300.0 | 600.0 | |||||
| Total material costs PCR/Qiaxcel and ddPCR method | 873.2 | 1,549.0 | 3,097.9 | ||||||
| BMA salary costs PCR/Qiaxcel and ddPCR method | 400.0 per hour | 6 | 2,400.0 | 8 | 3,200.0 | 12 | 4,800.0 | ||
| Total costs PCR/Qiaxcel and ddPCR method | 3,273.2 | 4,749.0 | 7,897.9 | ||||||
| Costs per sample PCR/Qiaxcel and ddPCR method | 3,273.2 | 678.4 | 526.5 | ||||||
All costs in Swedish crowns (SEK), 1 USD ≈ 8.5 SEK.
BMA, biomedical analyst; nc, negative control.