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. 2021 Oct 10;12:537. doi: 10.1186/s13287-021-02590-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of hASCs. A Phase contrast micrograph of in vitro cultured cells isolated from the adipose tissue biopsies. Scale bar = 100 μm. B Cytofluorimetric analyses showing absence of CD45 (left) and positivity for CD105, CD90 and CD73 (centre and right) in the putative hASCs obtained in culture. C Trilineage mesenchymal differentiation potential of hASCs into adipocytes (left), osteoblast (centre) and chondroblast (right), as confirmed through staining of lipid droplets with Oil Red O, mineralized matrix deposition with Alizarin Red S and sulphated proteoglycan deposition with Alcian Blue, respectively. Scale bar left and centre = 100 μm, scale bar right = 25 μm