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. 2021 Oct 10;12:537. doi: 10.1186/s13287-021-02590-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Follicle morphology, count and classification. A Representative micrographs of different categories of follicles containing oocyte present in sections of ovaries obtained from animals subjected to the indicated treatments. Scale bar = 50 μm. B, C Total number of follicles per ovary. Values are expressed as mean ± SD. Significance is calculated with t-test (w0) and with One-way ANOVA (w4) vs CTR (letters) and vs CH (asterisks) as described in "Methods". D, E Number of follicles per ovary in function of the maturation stage: primordial (p0), primary (I), secondary (II) and antral (A). Values are expressed as mean ± SD. Significance is calculated with Two-way ANOVA vs CTR (letters) and vs CH (asterisks) as described in "Methods". Sample size: n ≥ 3 and n ≥ 4 for light (L) and heavy (H) chemotherapeutic treatment, respectively