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. 2021 Oct 10;12:537. doi: 10.1186/s13287-021-02590-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Analysis of pre-implantation embryo development in vitro and of time to plug after natural mating. A, B, C After superovulation, females were mated, sacrificed at E1.5 and embryos collected from both ampullae were counted and classified. Two-cell embryos were cultured in vitro for 72 h to reach the blastocyst stage as seen in the representative micrographs in A (Scale bars = 100 μm). In B and C values per ampulla are expressed as means ± SD. Significance is calculated with t-test vs CTR (letters) and vs CH (asterisks) as described in "Methods". Sample size: n = 3 and n ≥ 10 for light (L) and heavy (H) chemotherapeutic treatment, respectively. D, E Days of natural mating required to achieve a vaginal plug. Mean values for the whole period analysed are shown on the left panels, while the trend of the mean values over time is shown on the right panels. Values are expressed as means ± SD. Significance is calculated with One and Two-way ANOVA, respectively, vs CTR (letters) and vs CH (asterisks) as described in "Methods". Sample size: n = 3 and n ≥ 7 for light (L) and heavy (H) chemotherapeutic treatment, respectively