Fig. 2.

Reduced expression of ACE2 and NRP1 is mediated by blocking histone deacetylases. HK-2 and Huh-7 cell lines were treated with different histone deacetylase (HDAC) inhibitors; trichostatin A (TSA, specific for class I and II HDACs, 200 and 400 nM), PCI-24781 (specific for class I and IIb HDACs, 1 and 2 µM), TMP-195 (specific for class IIa HDACs, 5 and 10 µM) and sirtinol (specific for SIRT1 and SIRT2, a class III HDAC, 5 and 10 µM) for 24 h. All inhibitors were prepared in DMSO, which was also used as control. Expression of the ACE2 and NRP1 receptors was assayed by WB using β-actin as endogenous control. Data are presented as the mean ± SD of at least three independent experiments. *p < 0.05