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. 2021 Oct 11;14:149. doi: 10.1186/s13041-021-00851-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Procedure for the neuronal differentiation and characterization of hiPSC-NPCs. a Procedure for the neuronal differentiation of hiPSC-NPCs. The phase-contrast image shows the neurosphere of the hiPSC-NPCs before differentiation, and the immunofluorescence image shows neurons derived from hiPSC-NPCs (green: βIII-tubulin, blue: DAPI). Scale bars: 200 μm and 20 μm. b Gene expression of hiPSC-NPCs (1210B2 and 1201C1) and human neural tissue-derived NSPCs (oh-NSC-3-fb and oh-NSC-8-sp) before differentiation. The ΔCt values are shown. Red: high expression. UD undetermined