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. 2021 Oct 11;14:149. doi: 10.1186/s13041-021-00851-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Phenotypic analysis of hiPSC-neurons on day 49 of differentiation. a Immunofluorescence analysis of glutamatergic neurons (VGLUT1 and VGLUT2), dopaminergic neurons (TH), GABAergic neurons (GAD67), cholinergic neurons (VAChT and ChAT), and glial cells (GFAP) on day 49 of differentiation in 1210B2 cells. The nuclei were stained with DAPI (blue). Scale bar: 20 μm. b, c Comparison of the gene expression levels of VGLUT1 (b) and VGLUT2 (c) in hiPSC-derived neurons (1210B2 and 1201C1, both without NGF) on day 49 and in adult human samples (cerebellum and cerebrum). UD undetermined