Figure S4.

RT-qPCR of putative PDCD4 target genes. Relating to Fig. 6. (A) RT-qPCR of putative PDCD4 target genes, Scd1 and Thrsp, from TTX-silenced and Bic-stimulated neurons that were transduced with PDCD4 WT or S71A from six sets of cultures. Samples were normalized using two housekeeping genes, Hprt and Gapdh. The abundance of the target gene in each Bic sample was normalized to its respective TTX sample. Scd1 WT CHX median = 1.702; Scd1 S71A CHX median = 0.5776; Scd1 WT median = 2.401; Scd1 S71A median = 0.7672; Thrsp WT CHX median = 1.826; Thrsp S71A CHX median = 0.9080; Thrsp WT median = 1.522; Thrsp S71A median = 0.5995. Scd1 CHX WT versus CHX S71A, P = 0.0022; Scd1 WT versus S71A, P = 0.0260; Thrsp CHX WT versus CHX S71A, P = 0.0043; Thrsp WT versus S71A, P = 0.0043. (B) RT-qPCR of Pdcd4, Scd1, and Thrsp from PDCD4 knockdown experiments in TTX-silenced neurons from seven sets of cultures. Samples were normalized to the housekeeping gene Gapdh. All values were normalized to the median value of the control condition (control siRNA). Pdcd4 control siRNA median = 1.00; Pdcd4 PDCD4 siRNA median = 0.51. Pdcd4 control versus PDCD4 siRNA, P = 0.0006. Scd1 control siRNA median = 1.00; Scd1 PDCD4 siRNA median = 3.77. Scd1 control versus PDCD4 siRNA, P = 0.0111. Thrsp control siRNA median = 1.00; Thrsp PDCD4 siRNA median = 2.08. Thrsp control versus PDCD4 siRNA, P = 0.0023. (C) RT-qPCR of Pdcd4, Scd1, and Thrsp from PDCD4 overexpression experiments in TTX-silenced neurons from seven sets of cultures. Samples were normalized to the housekeeping gene Gapdh. All values were normalized to the median value of the control condition (no AAV). Pdcd4 no AAV median = 1.00; Pdcd4 PDCD4 AAV median = 11.93. Pdcd4 no AAV versus PDCD4 AAV, P = 0.0006. Scd1 no AAV median = 1.00; Scd1 PDCD4 AAV median = 0.80. Scd1 No AAV versus PDCD4 AAV, P = 0.3176. Thrsp no AAV median = 1.00; Thrsp PDCD4 AAV median = 0.57. Thrsp no AAV versus PDCD4 AAV, P = 0.0262. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Mann-Whitney U test.