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. 2021 Sep 19;69(10):659–667. doi: 10.1369/00221554211047287

Figure 1.

Figure 1.

Effect of antigen retrieval modifications on immunodetection. A: Variation in fluorescence intensity (expressed as channel value for 90% of the positive pixels) for each marker according to the four AR treatments: EDTA, EDTA + Lactose (LACT), EDTA + SDS (SDS), and EDTA + Lactose + SDS (FULL). Fourteen samples tested for each marker, except Ki-67 (13 samples) and CD34 (4 samples). B: Comparison of variation in fluorescence intensity (expressed as channel value for 90% of the positive pixels) between EDTA and FULL AR for each marker. Fourteen samples tested for each marker except CD34 (4 samples). Abbreviations: AR, antigen retrieval; SDS, sodium dodecyl sulfate; AF, autofluorescence.