Table 1.
Primary and Secondary Antibodies.
| Primary Antibodies | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Clone | Isotype | Cat. No. | Source | Concentration | Dilution | Lot | RRID_AB | AR Preferred pH |
| BCL-2 | Bcl-2-100 | Mo IgG1 | B3170-.2ML | Merck Sigma Aldrich | 5.1 mg/ml | 1 µg/ml | 022M4805 | 258541 | High |
| Blimp-1/PRDM1 | 6D3 | Rat IgG2a | sc-47732 | SCBT | 200 µg/ml | 1 µg/ml | NA | 628168 | High |
| CD4 | EPR6855 | Rb Mab | ab133616 | Abcam/Epitomics | 166 µg/ml | 1 µg/ml | GR3215096 | 2819211 | High |
| CD14 | HPA002127 | Rb Ab | HPA002127 | Merck Sigma Aldrich | 400 µg/ml | 1 µg/ml | A113974 | 1078440 | Low |
| CD34 | 43A1 | Mo IgG3 | sc-65261 | SCBT | 100 µg/m1 | 1 µg/ml | A1212 | 631133 | High |
| Cytokeratin 19 | A53-B/A2 | Mo IgG2a | sc-6278 | SCBT | 200 µg/ml | 1 µg/ml | H0614 | 627851 | High |
| HLA-DR | SPM288 | Mo IgG2b | sc-56545 | SCBT | 200 µg/ml | 1 µg/ml | B0909 | 1125217 | High |
| IRF4 | IRF-4 (M-17) | Goat | sc-6059 | SCBT | 100 µg/ml | 1 µg/ml | J2015 | 2127145 | High |
| Ki-67 | 4A1 | Mo IgG2b | SAB5300423 | Merck Sigma Aldrich | NA | 1:200 | 12110662 | 10980978 | High |
| MLH1 | ES05 | Mo IgG1 | M364029 | Dako | 91.6 µg/ml | 1 µg/ml | 10053229 | 2631352 | High |
| SOX9 | CL0639 | Mo IgG2a | AMAB90795 | Merck Sigma Aldrich | 1000 µg/ml | 1 µg/ml | 2712 | 2665670 | NA |
| Vimentin | V9 | Mo IgG1 | M0725 | Dako | 156 µg/ml | 1 µg/ml | 83947 | 83947 | High |
| Secondary Antibodies | |||||||||
| Name | Notes | Cat. No. | Source | Concentration | Dilution | Lot | RRID_AB | ||
| Brilliant Violet 480 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific* | Fc-specific | 115-685-206 | Jackson Immunoresearch | 0.2 mg/ml | 1:100 | 130919 | 2651097 | ||
| Alexa Fluor 488 Goat Anti-Mouse, Fcγ Subclass IgG1 Specific* | Fc-specific | 115-545-205 | Jackson Immunoresearch | 1.6 mg/ml | 1:200 | 145537 | 2338854 | ||
| Rhodamine Red-X (RRX) AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific* | Fc-specific | 115-295-207 | Jackson Immunoresearch | 1.8 mg/ml | 1:200 | 118791 | 2338771 | ||
| Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG** | H+L | 711-605-152 | Jackson Immunoresearch | 1.5 mg/ml | 1:200 | 138314 | 2492288 | ||
| PerCp AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific* | Fc-specific | 115-125-209 | Jackson Immunoresearch | 0.5 mg/ml | 1:200 | 88606 | 2338635 | ||
| Brilliant Violet 480 AffiniPure Donkey Anti-Rabbit IgG (H+L)** | H+L | 711-685-152 | Jackson Immunoresearch | 0.2 mg/ml | 1:100 | 136043 | 2651109 | ||
| Alexa Fluor 488-AffiniPure Donkey Anti-Goat IgG (H+L)** | H+L | 705-545-147 | Jackson Immunoresearch | 1.5 mg/ml | 1:200 | 139173 | 2336933 | ||
| Rhodamine Red-X (RRX) AffiniPure Donkey Anti-Mouse IgG(H+L)* | H+L | 715-295-151 | Jackson Immunoresearch | 1.5 mg/ml | 1:200 | 117959 | 2340832 | ||
| Alexa Fluor 647 AffiniPure Donkey Anti-Rat IgG (H+L)** | H+L | 712-605-153 | Jackson Immunoresearch | 1.5 mg/ml | 1:200 | 133374 | 2340694 | ||
Secondary antibody absorption
*
(min X Hu, Bov, Rb Sr Prot).
**
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot).