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. 2021 Aug 25;27(6):437–447. doi: 10.1177/17534259211035426

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Wolf–Hirschhorn syndrome candidate 1 (WHSC1) was up-regulated in LPS-induced acute lung injury (ALI). (a) Hematoxylin and eosin (HE) stain was conducted to assess lung tissue damage in mice treated with 5 or 10 mg/kg LPS. (b) The lung injury score was determined to evaluate lung injury in mice treated with LPS. (c) Levels of IL-18 and IL-1β in bronchoalveolar lavage fluid (BALF) were detected by ELISA assay. (d) The lung wet/dry mass ratio of lung tissue was measured in mice treated with LPS. (e) The level of BALF total protein was determined in mice treated with LPS. (f) The mRNA and protein levels of WHSC1 in alveolar macrophages of mice treated with LPS were detected by quantitative RT-PCR and Western blot.