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. 2021 Aug 25;27(6):437–447. doi: 10.1177/17534259211035426

Figure 2.

Figure 2.

Knockdown of WHSC1 attenuated LPS-induced ALI. (a) The mRNA and protein levels of WHSC1 were detected by quantitative RT-PCR and Western blot in alveolar macrophages of mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS. (b) The lung tissue damage in mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS was determined by HE stain. (c) The lung injury score was determined to evaluate lung injury in mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS. (d) The levels of IL-18 and IL-1β in BALF of mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS were detected by ELISA assay. (e) The lung wet/dry mass ratio of lung tissues in mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS was determined. (f) The BALF total protein level was determined in mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS.