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. 2021 Aug 25;27(6):437–447. doi: 10.1177/17534259211035426

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Knockdown of NEK7 enhanced the inhibitory effect of WHSC1 silencing on LPS-induced alveolar macrophage pyroptosis. (a) The expression level of NEK7 was determined using quantitative RT-PCR and Western blot in alveolar macrophages transfected with siRNA targeting WHSC1 and treated with 1 μg/ml LPS. (b) The expression level of NEK7 was determined using quantitative RT-PCR and Western blot in alveolar macrophages of mice introduced into Ad-shWHSC1 and treated with 10 mg/kg LPS. (c) A co-immunoprecipitation assay was performed to explore the relationship between NEK7 and NLRP3. (d) The protein levels of WHSC1, NEK7, NLRP3, ASC, caspase-1 p20, IL-18, IL-1β, GSDMD FL and GSDMD CL were determined using Western blot in alveolar macrophages transfected with siRNAs targeting WHSC1 and NEK7 and treated with 1 μg/ml LPS. Ns: P > 0.05.