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. 2021 Aug 25;27(6):437–447. doi: 10.1177/17534259211035426

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — The effect of WHSC1 and NEK7 on alveolar macrophage pyroptosis. (a) The expressions of caspase-1 and GSDMD CL in alveolar macrophages transfected with siRNAs targeting WHSC1 and NEK7 and treated with 1 μg/ml LPS were detected using immunofluorescence. (b) The activity of caspase-1 in alveolar macrophages was determined in alveolar macrophages transfected with siRNAs targeting WHSC1 and NEK7 and treated with 1 μg/ml LPS. (c) The pyroptosis of macrophages in alveolar macrophages transfected with siRNAs targeting WHSC1 and NEK7 and treated with 1 μg/ml LPS was assessed by flow cytometry. (d) The pyroptosis proportion of macrophages in alveolar macrophages transfected with siRNAs targeting WHSC1 and NEK7 and treated with 1 μg/ml LPS is presented.