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. 2021 Aug 25;27(6):437–447. doi: 10.1177/17534259211035426

Figure 7.

Figure 7.

Enforced NEK7 reversed the inhibitory effect of WHSC1 silencing on LPS-induced alveolar macrophage pyroptosis. (a) The expression level of NEK7 was determined using Western blot in alveolar macrophages co-transfected with NEK7A overexpression vector and siRNAs targeting WHSC1 and treated with 10 mg/kg LPS. (b) The protein levels of WHSC1, NEK7, NLRP3, ASC, caspase-1 p20, IL-18, IL-1β and GSDMD CL were determined using Western blot in alveolar macrophages co-transfected with NEK7A overexpression vector and siRNAs targeting WHSC1 and treated with 10 mg/kg LPS. (c) The activity of caspase-1 in alveolar macrophage was determined in alveolar macrophages co-transfected with NEK7A overexpression vector and siRNAs targeting WHSC1 and treated with 10 mg/kg LPS. (d) The expressions of caspase-1 and GSDMD CL in alveolar macrophages co-transfected with NEK7A overexpression vector and siRNAs targeting WHSC1 and treated with 10 mg/kg LPS were detected using immunofluorescence. Ns: P > 0.05.