Figure 3.

The K694R polymorphism does not affect cell-surface TLR4 expression. (a) Representative flow cytometric identification of HEK293T cells transiently transfected with K694R or WT TLR4 plasmid, treated with or without LPS for 6 h, respectively, gated on GFP+ cells. The cell-surface TLR4 was stained with the anti-TLR4 Ab. Anti-mouse IgG (H+L), F(ab′)2 Fragment (Alexa Fluor® 647 Conjugate) was used as the second Ab. (b) Quantification of cell-surface TLR4 stained with anti-TLR4 Ab in GFP+ cells in the Q2 quadrant. Graphs are the mean ± SD, n = 6, *P < 0.05, Student’s t-test.