Figure 4.

The K694R polymorphism does not affect total TLR4 levels. (a) Representative flow cytometric identification of HEK293T cells transiently transfected with K694R or WT TLR4 plasmid, treated with or without LPS for 6 h, respectively, gated on GFP+ cells. Total TLR4 was stained with anti-TLR4 Ab. Anti-mouse IgG (H+L), F(ab′)2 Fragment (Alexa Fluor® 647 Conjugate) was used as the second Ab. (b) Quantification of total TLR4 stained with anti-TLR4 Ab in GFP+ cells in the Q2 quadrant. Graphs are the mean ± SD, n = 6, *P < 0.05, Student’s t-test.