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. 2020 Jun 8;27(6):483–492. doi: 10.1177/1753425920927479

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 7. — The K694R polymorphism impairs NF-κB activation and production of TNF-α, IL-1β, IL-6 and IL-18. (a) HEK293T cells transiently transfected with WT or K694R TLR4 plasmids were co-transfected with Flag-MD2, CD14, pNFκB-luc and pRL-SV40-N. Cells were treated with LPS for 6 h, and cell lysates were analysed for firefly and Renilla luciferase activities. Graphs are the mean ± SD, n = 6, ****P < 0.0001, Student’s t-test. (b) mRNA expression levels for the genes TNF-α, IL-1β, IL-6 and IL-18 in HEK293T cells transiently transfected with WT or K694R TLR4 plasmids and treated with LPS for 6 h were measured by quantitative RT-PCR. Graphs are the mean ± SD, n = 6, *P < 0.0001, Student’s t-test.