Figure 1.

OTX2 stimulates axon regeneration from RGCs in vitro.

NF-200 immunolabeling dissociated cultures from adult mouse retina shows a few cells with axons (red arrows) after 6 DIV (A), while medium supplemented with OTX2 stimulates long axon regeneration (B). The accumulative length of axon growing from dissociated adult RGCs was significantly increased after treatment with 0.66, 1.65 and 3.3 nM OTX2 compared to control (C). Images of live RGCs purified from adult rat retina show increased neurite growth when treated with 3.3 nM OTX2 (E) compared to control (D). The percent of live RGCs with neurites was significantly greater after treatment with 3.3 nM OTX2 than vehicle (control) (F). Comparisons were made by one-way analysis of variance followed by Fisher’s protected least significant difference for post hoc comparisons. *P < 0.05. n = 3–4 coverslips per condition. Scale bars: 100 µm. RGC: Retinal ganglion cells.