Figure 2.

OTX2 stimulates axon growth from explants of adult retina.

Adult rat retinal explants growing on laminin/poly-D-lysine-coated coverslips and labeled with NF-200 show numerous axons/fascicles (red arrows) when treated with 13.2 nM OTX2 (B) compared to vehicle (A). The accumulative length of the axons/axon fascicles was significantly greater after treatment with 52.8 nM OTX2 than with other concentrations of OTX2 (C). Explants from adult mouse retina cultured on poly-D-lysine alone have much less cumulative axon growth but have significantly more regrowth after treatment with 13.2 and 26.4 nM OTX2 than treatment with other concentrations of OTX2 (D). Comparisons were made by one-way analysis of variance (ANOVA) followed by Fisher’s protected least significant difference for post hoc comparisons. *P < 0.05. n = 4–5 explants per condition. Scale bars: 200 µm.