Skip to main content
. 2021 Aug 4;17(3):632–642. doi: 10.4103/1673-5374.320993

Figure 5.

Figure 5

The effect of CSF on primary hippocampal NSC proliferation and differentiation into neurons exposed to a high concentration of CORT.

(A) Cell viability as detected by Cell Counting Kit-8 (n = 10 per group). Cell viability (%) = [ODexperimental– ODblank]/[ODcontrol– ODblank] × 100. (B) BrdU/DCX double-positive cells (arrows). Normal CSF promoted NSC proliferation and differentiation. Compared with the CUMS-CSF group, the ratio of BrdU/DCX double-positive cells to total cells in the ICA-CSF group was significantly increased. BrdU: red, AlexaFluor®594; DCX: green, AlexaFluor®488; DAPI: blue. Scale bars: 500 μm; original magnification, 10×; 50 μm in enlarged images. (C) The number of BrdU-positive cells (n = 4–7 per group). (D) Ratio of BrdU/DCX double-positive cells to total cells (n = 4–7 per group). Data are expressed as the mean ± SEM, and were analyzed by one-way analysis of variance followed by the least significant difference (A, D) or Games-Howell (C) post hoc test. *P < 0.05, **P < 0.01. BrdU: 5-Bromo-2-deoxyuridine; CON: control; CORT: corticosterone; CSF: cerebrospinal fluid; CUMS: chronic unpredictable mild stress; DAPI: 4′,6-diamidino-2-phenylindole; DCX: doublecortin; FBS: fetal bovine serum; ICA: cerebrospinal fluid; n.s.: not significant; NSC: neural stem cell.