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. Author manuscript; available in PMC: 2021 Oct 11.
Published in final edited form as: Nat Struct Mol Biol. 2021 Jun 11;28(6):501–511. doi: 10.1038/s41594-021-00604-7

Extended Data Fig. 6 |. Transcription is not required for polycomb redistribution.

Extended Data Fig. 6 |

a, Experimental design to test whether polycomb redistribution requires transcription. b, qRT-PCR depicting fold change by ΔΔCt method with indicated treatments from n = 3 biological replicates. c,d, Scatterplot showing that Ring1b and Suz12 changes following Brg1 degradation are highly correlated despite global inhibition of transcription for all peaks c, and peaks that were significantly changed by Brg1 degradation alone (d) (FDR < 0.1). e, Scatterplot of changes to Ring1b and Suz12 upon treatment with auxin (auxin/control) or triptolide (triptolide/control) showing distinct effects from the two treatments. Correlation and p-values were obtained from Pearson’s product moment correlation for c-e, from n = 4 biological replicates at the 8 h timepoint.