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. Author manuscript; available in PMC: 2021 Oct 11.
Published in final edited form as: Cancer Res. 2016 Apr 7;76(10):2932–2943. doi: 10.1158/0008-5472.CAN-15-3332

Figure 3. Age affects tumor stroma.

Figure 3.

(A) Derivation of granulin (GRN)-dependent and GRN-independent cancer associated fibroblasts (CAFs) from normal human mammary fibroblasts (hMFs). (B) Table shows select genes upregulated in GRN-dependent v GRN-independent CAFs (GEO GSE75333) (C) Collagen IV (red, left) and PDGFR (red, right) staining in the distant tumors from young and old mice. Cell nuclei counterstained with hematoxylin. Scale bar=100 μm. (D) Ki67 (brown) staining in distant tumors from young and old mice; cell nuclei counterstained with hematoxylin. Dashed lines segregate tumor (T) from stroma; arrows indicate proliferating stromal cells. Scale bar=25 μm.