FIGURE 2.

Growth characteristics of Ralstonia jumbo phage RsoM2USA. (A) One-step growth curve of the phage with R. solanacearum strain RUN302 as the host. The phage was added at a MOI of 1 and allowed to adsorb for 15 min at 28°C. Phage titers were determined every 30 min using the plaque assay. The latent period is when no release of phage particles was observed. The rise period begins with the end of the latent period and ends when the increase in phage titers ceases. The burst size is the average number of phage particles released per infected cell. (B) Effect of temperature on the stability of phage RsoM2USA. 10⁸ PFU of phage RsoM2USA was incubated at each temperature point and the number of phage was estimated by plaque assay using R. solanacearum RUN302 as a host 1 h after incubation. Means are based on three separate experiments, each containing three replicates. Bars indicate standard deviations.