FIGURE 7.

The deletion of Nrf2 reversed the reduced mRNA expression of pro‐inflammatory cytokines and increased the mRNA level of anti‐inflammatory cytokines induced by TDZD administration after ischemia/reperfusion. (A–C) The mRNA expression of pro‐inflammatory cytokines (TNF‐α, IL‐1β, and iNOS) following 7 days of reperfusion. (D–F) The mRNA levels of anti‐inflammatory factors (CD‐206, YM1/2, arginase‐1) 7 days after reperfusion. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 versus the I/R group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus the I/R group; ^p < 0.05, ^^p < 0.01, ^^^p < 0.001. One‐way ANOVA with Tukey's post hoc test was used for statistical analysis. AAV‐Nrf2, adeno‐associated virus induced Nrf2‐shRNA; I/R, ischemia/reperfusion; IL‐1β, Interleukin‐1β; iNOS, inducible nitric oxide synthase; TDZD, 4‐Benzyl‐2‐methyl‐1,2,4‐thiadiazolidine‐3,5‐dione; TNF‐α, Tumor necrosis factor‐α