Table 1.
Sequences of the primers used in this study.
| Primer | Sequences (5'-3') | Primer information |
|---|---|---|
| SIRT1-F1 | AAGATGGCGGACGGAGAGAG | RT primer |
| SIRT1-R1 | ATTTAAAGGTGTGTGGTGCTC | RT primer |
| SIRT1-5-F1 | CCCAGTCCATTGTTGTCTCCGCCT | 5'RACE primer (inner) |
| SIRT1-5-F2 | TCCTCAGTTGTTCCAAGCATAGCG | 5'RACE primer (outer) |
| SIRT1-3-R1 | CCATAGTGTGATGTCAGCTGTGGT | 3'RACE primer (inner) |
| SIRT1-3-R2 | ACGGCATACCATGAATTCAGCACT | 3'RACE primer (outer) |
| SIRT1-F | TGGATTGTGAGGCTGTAAGG | qPCR |
| SIRT1-R | ATGAGGAATGGAGTTTGGGA | qPCR |
| Sirt1-Ecorl-F | GGATCCACTAGTCCAGTGTGGTGGAATGGCGGACGGAGAGAGC | Construction of expression plasmid |
| Sirt1-Ecorl-R | GCCACTGTGCTGGATATCTGCAGAAAAGGTGTGTGGTGCTCTGAGT | Construction of expression plasmid |
| Nrf2-F | CACCAAAGACAAGCGTAAG | RT-qPCR |
| Nrf2-R | GAAATCATCAACAGGCAGA | RT-qPCR |
| PPARα-F | CCACCGCAATGGTCGATATG | RT-qPCR |
| PPARα-R | TGCTGTTGATGGACTGGGAAA | RT-qPCR |
| Gpx-F | CTCCTCAACCAGGCAAAC | RT-qPCR |
| Gpx-R | ATACCCCCCTCACAACAA | RT-qPCR |
| CAT-F | TGAATGGCTATGGCTCTC | RT-qPCR |
| CAT-R | AATCTGGGTTGGTGGAAG | RT-qPCR |
| SOD1-F | TTATTTTGAGCAGGAGGG | RT-qPCR |
| SOD1-R | TTCTTGTTGTGGGGATTG | RT-qPCR |
| SOD2-F | GGTCTCATTCCCCTTCTT | RT-qPCR |
| SOD2-R | TCGCTCACATTCTCCCAG | RT-qPCR |
| ATGL-F | GCTCCCCTACACTCTCCCTCT | RT-qPCR |
| ATGL-R | CTGCTCTCGAATCCACTCAAC | RT-qPCR |
| ACO1-F | GGAGGTTATTGTTTCGGTTCT | RT-qPCR |
| ACO1-R | GCCTTCTTTTGGTCTTTTCTG | RT-qPCR |
| CPT1-F | GAATGGGGTAATGACTGGTGTG | RT-qPCR |
| CPT1-R | TCGACTGATTGGTATGTGTTGG | RT-qPCR |
| SREBP-1-F | TGGGGATTGGGCTATTCG | RT-qPCR |
| SREBP-1-R | CCTGGCTGGGGCTTTTCT | RT-qPCR |
| FAS-F | AGGCTGAGTGGGAGAAGGTG | RT-qPCR |
| FAS-R | GACGGCGACAAAGAAAGAGG | RT-qPCR |
| ACC1-F | ATCCCTCTTTGCCACTGTTG | RT-qPCR |
| ACC1-R | GAGGTGATGTTGCTCGCATA | RT-qPCR |
| ACC2-F | GACAGAGAGTGATTCAGGTGGA | RT-qPCR |
| ACC2-R | GCTGGTTGTTGGAAGTGTAGAC | RT-qPCR |
| β-actin-F | AAAGGGAAATCGTGCGTGAC | RT-qPCR |
| β-actin-R | AAGGAAGGCTGGAAGAGGG | RT-qPCR |