WY-14,643 mixed in ethanol diets induces PPARα pathway and inhibits hepatic TG accumulation induced by ethanol alone or in combination with nicotine. (A) Western blotting analyses for PPARα-regulated ACOX, L-FABP, FGF21, and CYP4A expression in liver. (B) Serum levels of FGF21. (C) Liver contents of TG. (D) liver contents of FFA. (E) Serum levels of TG. (F) Serum levels of FFA. Cont, Control group; E, Ethanol group; EWY, Ethanol/WY-14,643 group; EN, Ethanol/nicotine; ENWY, Ethanol/nicotine/WY-14,643.