Fig. 5. Graphical summary. DNA methylation results reveal hypomethylation of SLFN11 promoter region in HCC1937 BRCA1 mutant cells. This combination has been shown to impact clinical treatment options. DNMT3A is a DNA methyltransferase and was elevated in HCC1937 cells along with Histone H3K36me. NSD2 is a histone methyltransferase elevated in MDA-MB-231 cells and is known to convert H3K36 to H3K36me1/2; transforming heterochromatin into euchromatin (active state). H3K36me2 recruits DNMT3A to shape the intergenic DNA methylation landscape. MDA-MB-231 cells showed increase TGFBR1, SMAD5 S465ph, and PTEN S385ph elevation leading to increased expression of TGFβ signaling and PI3K/AKT/mTOR pathways. HCC1937 cells had decreased expression of PTEN S385ph and decreased PI3K/AKT/mTOR and WNT/β-catenin pathways.