Fig. 1.

Summary of Strand-seq QC with ASHLEYS: (A) the Strand-seq protocol generates a large number of single-cell libraries that need to pass QC before downstream analysis. Strand-seq panels reused from Sanders et al. (2020; Fig. 1a). (B) Example for the Strand-seq specific feature of the Watson/Crick read distribution for high- (top) and low-quality (middle) libraries. ASHLEYS also evaluates library quality based on generic sequencing library features (bottom text). (C) Performance summary for the SVC model shipped with ASHLEYS for training and testing stages of model building. Feature names omitted in feature importance plot for improved readability (cf. Supplementary Fig. S1)