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. 2021 Sep 27;13:717644. doi: 10.3389/fnagi.2021.717644

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Copyright © 2021 Liu, Zheng, Xu, Cao, Wang, Wang, Zhao, Zhang and Liao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enriched environment decreased ischemic infarction and inhibited pyroptosis after I/R injury. (A) Schematic brain with a highlight of the ischemic border. (B,D) Representative cresyl violet stained brain slices and quantification of cerebral infarct volume percentage. Scale bars, 5000 μm. n = 6. (C,E) Protein levels of GSDMD-N in peri-infarct tissues. n = 6. (G,F) Representative IHC staining images for GSDMD in the penumbra and IHC score of GSDMD in the penumbra. Scale bars, 50 μm. n = 6. Data are expressed as mean ± SD. *p < 0.05, ***p < 0.001 vs. SSC group; ^##p < 0.01, ^###p < 0.001 vs. ISC group.