Fig 1.
(a) Schematic of PCR amplification of VH and VL cDNAs by signal peptide (SP) primers. Right panel shows light chain PCR, which typically results in amplification of myeloma cDNA by some primers (primarily SP2) and antigen specific cDNA by other SP primers. (b) DNA gels. For the heavy chain (left panel), SP4 gave the major product, which was sequenced and revealed a full-length VH cDNA. For the light chain (right panel), SP2 amplified the non-productive Sp2/0 myeloma kappa chain, whereas SP7 primer amplified a full-length VL cDNA.