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(a) Results of Edman sequencing of H5C6 hybridoma-derived mAb—summary, standards, and first three residues are shown. (b) Comparison of amino acid sequences of full-length SP2 VL cDNA and aberrant Sp2/0 myeloma sequence. Both the frameshift mutation and the conserved cysteine (C23Y) mutation are corrected in the full-length gene product.
