| Gene (Melolontha melolontha) |
Mm_bGlc17
|
This paper |
|
See Supplementary file 1
|
| Genetic reagent (Taraxacum officinale) |
TA-G-deficient plants |
Doi:10.1371/journal.pbio.1002332
|
A34-RNAi-1 |
|
| Genetic reagent (M. melolontha) |
Mm_bGlc17 silenced |
This paper |
|
See ‘Materials and methods’ (‘TA-G deglucosylation activity in RNAi-silenced M. melolontha larvae’) |
| Cell line (Trichoplusia ni) |
High Five Cells |
Life Technologies, Carlsbad, CA, USA |
|
|
| Transfected construct (T. ni) |
pIB/V5-His TOPO |
Life Technologies, Carlsbad, CA, USA |
|
|
| Biological sample (M. melolontha) |
M. melolontha
|
This paper |
|
Collection of different natural populations |
| Biological sample (T. officianle) |
T. officinale
|
Doi: |
|
Different natural genotypes |
| Recombinant DNA reagent |
Plasmid pGJ 2648 |
Other |
|
Supplied by Dr. Christian Schulze-Gronover, Fraunhofer Institute for Molecular Biology and Applied Ecology |
| Recombinant DNA reagent |
pCR2.1-TOPO plasmids |
Life Technologies |
|
|
| Recombinant DNA reagent |
cDNAs |
This paper |
|
See Supplementary file 3
|
| Sequence-based reagent |
Primers |
This paper |
|
See Supplementary file 2
|
| Sequence-based reagent |
KAPA SYBR FAST qPCR Master Mix |
Kapa Biosystems |
|
|
| Commercial assay or kit |
innuPREP RNA Mini Kit |
Analytik Jena, Jena, Germany |
|
|
| Commercial assay or kit |
RNeasy Plant Mini Kit; RNeasy Lipid Tissue Mini Kit |
Qiagen |
|
|
| Commercial assay or kit |
SMARTer RACE cDNA Amplification Kit |
Clontech, Mountain View, CA, USA |
|
|
| Commercial assay or kit |
FuGeneHD-Kit |
Promega, Madison, WI, USA |
|
|
| Commercial assay or kit |
GeneJET Gel Extraction Kit; DreamTaq DNA Polymerase; MEGAscript RNAi Kit |
Thermo Fisher Scientific, Waltham, MA, USA |
|
|
| Commercial assay or kit |
KAPA SYBR FAST qPCR Kit Optimized for LightCycler 480 |
Kapa Biosystems, Wilmington, MA, USA |
|
|
| Chemical compound, drug |
Bis(p-nitrophenyl)phosphate; castanospermine; acarbose |
Sigma Aldrich |
|
|
| Chemical compound, drug |
Blasticidin; Express Five culture medium |
Life Technologies |
|
|
| Chemical compound, drug |
x Protease Inhibitor HP Mix |
SERVA Electrophoresis, Heidelberg, Germany |
|
|
| Chemical compound, drug |
TA-G; TA; BXDs; 4-MSOB |
This paper |
|
See ‘Materials and methods’ (‘Enzymatic assays of recombinant proteins’ and ‘Synthesis of TA-G metabolite standards’) |
| Chemical compound, drug |
Salicin |
Alfa Aeser |
|
|
| Chemical compound, drug |
Cellobiose |
Fluka |
|
|
| Chemical compound, drug |
Glc-MU |
Sigma Aldrich |
|
|
