Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Organism (Bombus terrestris) | NA | Koppert, Netherlands | NATUPOL | NA |
| Organism (Megalopta genalis) | NA | Wild caught, Barro Colorado Island, Panama | NA | NA |
| Antibody | Synapsin (SYNORF1; Mouse, monoclonal) | Developmental Studies Hybridoma Bank (DHSB) |
3C11 | NA |
| Antibody | Serotonin (5HT; Rabbit, polyclonal) | ImmunoStar | CAT#: 20080; RRID:AB_572263 |
NA |
| Antibody | Tyrosine hydroxylase (TH; Mouse, monoclonal) | ImmunoStar | CAT#: 22941; RRID:AB_572268 |
NA |
| Antibody | AZniPure Donkey Anti-Mouse IgG (H+L) Cy3 (polyclonal) | Jackson ImmunoResearch | CAT#: 715-165-150; RRID:AB_2340813 |
NA |
| Antibody | AZniPure Donkey Anti-Rabbit IgG (H+L) Cy5 (polyclonal) | Jackson ImmunoResearch | CAT#: 711-175-152; RRID:AB_2340607 |
NA |
| Other (serum) | Normal goat serum | Jackson ImmunoResearch | CAT#: 005-000-001 | NA |
| Chemical compound | Neurobiotin | Vector Laboratories | CAT#: SP-1120 | NA |
| Chemical compound | Cy3 Streptavidin | Jackson ImmunoResearch | CAT#: 016-160-084 | NA |
| Chemical compound | Permount | Fisher Scienti1 | CAT#: SP15-100 | NA |
| Other (enzyme) | Pronase | Sigma-Aldrich | CAT#: P8811 | NA |
| Chemical compound | Osmium tetroxide 4% aqueous | Proscitech | CAT#: C011 | NA |
| Chemical compound | Potassium ferricyanide | Sigma-Aldrich | CAT#: 702587 | NA |
| Chemical compound | Thiocarbohydrazide | Sigma-Aldrich | CAT#: 223220 | NA |
| Chemical compound | Lead nitrate | Sigma-Aldrich | CAT#: 228621 | NA |
| Chemical compound | L-Aspartic acid | Sigma-Aldrich | CAT#: A4534 | NA |
| Chemical compound | Uranyl acetate | Electron Microscopy Sciences | CAT#: 22400 | NA |
| Chemical compound | Durcupan ACM resin (4-part component kit) | Sigma-Aldrich | CAT#: 44610 | NA |
| Chemical compound | Conductive silver epoxy (2-part) | Ted Pella | CAT#: 16043 | NA |
| Software, algorithm | Amira 5.3 | Thermo Fisher Scienti1c | NA | NA |
| Software, algorithm | Skeletonize plugin for Amira 5.3 | Dr. J.F. Evers Schmitt et al., 2004 |
NA | NA |
| Software, algorithm | CATMAID | Saalfeld et al., 2009 | NA | NA |
| Software, algorithm | FIJI (ImageJ) | Schindelin et al., 2012 | NA | NA |
| Software, algorithm | natverse | Bates et al., 2020 | NA | NA |
| Software, algorithm | Leica Application Suite X (LAS X) Navigator | Leica | NA | NA |
| Software, algorithm | ImageJ image stitching plugin | Preibisch et al., 2009 | NA | NA |
| Other | Sigma VP 300 SEM | Zeiss | NA | NA |
| Other | 3view 2 system | Gatan | NA | NA |
| Other | Leica SP8 DLS inverted confocal microscope | Leica | NA | NA |
| Other | Ampli1er (Panama): BA-03X | NPI | BA-03X | NA |
| Other | Ampli1er (Lund): SEC05-LX | NPI | SEC05-LX | NA |
| Other | Micromanipulator: SMXS-K-R | SensApex OY | SMXS-K-R | NA |
| Other | Electrode puller: P-97 | Sutter | NA | NA |
| Other | Borosilicate glass capillaries w/ filament | Sutter | BF150-75-10 | NA |