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. Author manuscript; available in PMC: 2021 Oct 11.
Published in final edited form as: Biotechnol Bioeng. 2020 Jul 9;117(10):3066–3080. doi: 10.1002/bit.27479

Figure 2. Cells surviving silica gel immobilization demonstrate enhanced survival upon re-immobilization relative to the original control population.

Figure 2.

Cells surviving immobilization for three days were extracted and cultured in 2-D for 1 week prior to immunostaining or re-immobilization. (A) Percentage of Ki67-positive cells (number of Ki67-expressing cells (red) normalized to number of DAPI-labeled cells (blue)) from immunofluorescence images (merged images shown) compared to 2-D control cells. Scale bar indicates 200 μm. (B) Fold change in viable cell number relative to Day 0 after re-immobilizing extracted cells in silica gels relative to cells immobilized for the first time (Control) (*P < 0.05 compared to “Control” for each timepoint).