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. 2021 Oct 4;2021:5712280. doi: 10.1155/2021/5712280

Figure 1.

Figure 1

miR-505-3p specifically binds to SIRT3. (a) Heat map of all differentially expressed circular RNAs between OA and normal chondrocyte samples. N = 6 (three different samples in each group). (b) Schematic illustration to show the overlapping of the potential target miRNAs of SIRT3, as predicted by TargetScan and miRNAs upregulated in OA identified by sequencing analysis. (c) The relative levels of 4 miRNA candidates in the human articular chondrocytes were examined by qRT-PCR. (d) The potential binding sites of miR-505-3p and SIRT3. (e) Luciferase reporter assay was performed to detect the luciferase activities of HEK-293T cells cotransfected with a luciferase reporter construct containing wild-type or mutant SIRT3 and miR-505-3p mimics or mimics NC. (f) The relative expression of SIRT3 in human articular chondrocytes treated with lentivirus-miR-505-3p or lentivirus vector was examined by qRT-PCR (data are presented as the mean ± SD, ∗P < 0.05 vs. control or as indicated by Student's t-test).