Table 2.
Number of glycoforms assigned by DDA and DIA for SARS2-CoV-2 spike protein in at least two replicates. Values in parentheses represent the total number of glycoforms detected, including those that were identified in only a single replicate
| Spike protein glycosylation sites | Glycoforms identified by DDA | Glycoforms identified by DIA |
|---|---|---|
| 17-NLTT | 1 (2) | 0 (0) |
| 61-NVTW | 2 (4) | 2 (5) |
| 74-NGTK | 50 (67) | 46 (94) |
| 122-NATN | 57 (73) | 68 (131) |
| 149-NKSW | 2 (2) | 1 (3) |
| 165-NCTF | 19 (24) | 5 (6) |
| 234-NITR | 5 (9) | 16 (34) |
| 282-NGTI | 42 (63) | 35 (61) |
| 331-NITN | 22 (29) | 69 (129) |
| 343-NATR | 53 (90) | 76 (169) |
| 603-NTSN | 9 (14) | 3 (14) |
| 616-NCTE | 19 (29) | 5 (19) |
| 657-NNSY | 17 (20) | 14 (36) |
| 709-NNSI | 23 (36) | 34 (80) |
| 717-NFTI | 25 (54) | 38 (96) |
| 801-NFSQ | 34 (52) | 35 (84) |
| 1074-NFTT | 30 (42) | 50 (82) |
| 1098-NGTH | 79 (124) | 114 (188) |
| 1134-NNTV | 11 (16) | 5 (13) |
| 1158-NHTS | 1 (3) | 0 (0) |
| 1173-NASV | 6 (8) | 4 (14) |
| 1194-NESL | 11 (15) | 3 (9) |
| Total | 73 (118) | 183 (458) |