Fig. 4.

Effects of the Tat-CIAPIN1 protein on cytokine-induced apoptotic signaling in RINm5F β-cells. We pretreated the cells with the Tat-CIAPIN1 protein (3 μM) for 1 h and then treated them with cytokines (5 ng/ml IL-1β, 10 ng/ml TNF-α, and 10 ng/ml IFN-γ). Then, we assessed the indicated protein expression levels (A, B) by Western blotting and measured the band intensity by densitometer. *P < 0.01 compared with cytokine-treated cells. We repeated all experiments at least three times, and present data as mean ± SEM.