Fig. 1. Generation and molecular characterisation of the Grin1 inducible-rescue mouse line.

a Targeting construct schematic and recombination events at the Grin1 locus in the generation of the Grin1^flneo/flneo mouse model. PGK, PGK promotor; neo, neomycin selection cassette; polyA, polyadenylation sequence. b Schematic of expected molecular events at the Grin1 locus. c Visualisation of Grin1 mRNA in mouse sagittal sections (20 µm) via fluorescent in situ hybridisation using Grin1 probes (left: whole brain; middle: 20× micrograph of prefrontal cortex). At right, sagittal sections (20 µm) of prefrontal cortex visualising GluN1 protein expression via fluorescent immunohistochemistry with a rabbit anti-GluN1 antibody (in-house, 1:200; secondary anti-rabbit Alexa 568) in WT, Grin1^KD, and Grin1^RESCUE mice.