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. 2020 Oct 13;26(7):2964–2976. doi: 10.1038/s41380-020-00910-4

Fig. 2. The growth of small human cerebral organoids after transplantation in vivo.

Fig. 2

a After 30 days postdifferentiation, human cerebral organoids were labeled with PGK-GFP. Ten days later, GFP+ cerebral organoids were grafted into the brains of immunodeficient mice and harvested at the indicated MPT (month posttransplantation) for histological analysis (1 MPT: n = 5 brains; 3 MPT: n = 8 brains). b Immunofluorescence staining for GFP and human nuclei (hN) demonstrating that the implant survived well and was distributed throughout the prefrontal cortex by 1 MPT. c One month later, the expression of SOX2, FOXG1, STEM121 (a human neurite marker), hN, NCAM (a human neural cell adhesion molecule), NEUN, and TUJ1 were detected in the mouse brain. d, e More than 2 months later, the expression of TBR1, FOXP2, SATB2, BRN2, and glutamate (GLU) indicated that cortical excitatory features were conserved in vivo. In addition, a representative image of immunostaining for GFP and human-specific synaptophysin (hSyn) after 3 MPT is shown. Scale bars: 100 μm.