Fig. 6.

Knockdown of ARHGAP5-AS1 promotes TGF-β signaling via SMAD7. A Immunofluorescence of SMAD7 with FITC-conjugated antibody was performed to detect location of SMAD7 in MDA-MB-231 cells. B Nuclear and cytoplasmic SMAD7 was detected by western blot, LAMIN B and GAPDH were used as loading controls. C Effects of ARHGAP5-AS1 knockdown on the activation of TGF-β signaling were detected by western blot. D Effects of SMAD7 knockdown on the activation of TGF-β signaling were detected by western blot. E Luciferase reporter assays were performed in 293T cells with indicated transfections. The error bars in all graphs represented SD and each experiment was repeated three times independently. *p < 0.05, **p < 0.01, ***p < 0.001