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. 2021 Aug 28;23:107–123. doi: 10.1016/j.omto.2021.08.013

Figure 5.

Figure 5

OA reduces HGPRT and 5′-NT in the PSP by activating lysosomal proteolysis

(A) Reverse transcription-quantitative polymerase chain reaction (RT-PCR) time course showing the impact of OA treatment (200 μM) on HPRT1 and NT5E transcription in A549 and MDA-MB-231 cells. (B) The influence of OA (200 μM) on HGPRT and 5′-NT degradation when using cycloheximide (CHX) (50 μg/mL) to block protein synthesis in A549 cells. The curves on the right side of the western blot images indicate the quantification of protein levels. (C) The impact of lysosome inhibitor chloroquine (CQ) (0.02 μM) and the proteasome inhibitor MG132 (0.01 μM) on the degradation of HGPRT and 5′-NT induced by OA (200 μM) in A549 cells. The curves on the right side of the western blot images indicate the quantification of protein levels. (D) The influence of two lysosome inhibitors, CQ (0.02 μM) and bafilomycin A1 (BAF) (0.08 μM), on HGPRT and 5′-NT degradation induced by OA (200 μM) in A549 cells. The curves on the right side of the western blot images indicate the quantification of protein levels. Error bars represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Student’s t test.