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. 2021 Aug 28;23:107–123. doi: 10.1016/j.omto.2021.08.013

Figure 7.

Figure 7

The PSP is overactivated in human lung cancer and breast cancer, with a negative linkage to patient survival

(A) Western blot showing HGPRT and 5′-NT expression in tumorous and paired normal adjacent tissues from patients with lung adenocarcinoma (n = 5). (B) Representative immunohistochemistry stained images of lung tissue microarrays using HGPRT and 5′-NT antibodies from samples obtained from patients with lung cancer (n = 34). Scale bars for ×40 images, 625 μm; scale bars for ×400 images, 50 μm. The midlines of the boxplots represent the median values of the data, with the upper and lower limits of the box indicating the third and first quartiles and the whiskers of the boxplot up to 1.5 times the interquartile ranges. The p values were calculated with non-parametric Wilcoxon rank-sum tests. N, paired normal adjacent lung tissues; T, tumorous lung tissues. (C) Representative immunohistochemistry stained images of lung tissue microarrays using HGPRT and 5′-NT antibodies in samples from patients with breast cancer (n = 20). Scale bars for ×40 images, 625 μm; scale bars for ×400 images, 50 μm. The midlines of the boxplots represent the median value of the data, with the upper and lower limits of the box indicating the third and first quartiles and the whiskers up to 1.5 times the interquartile ranges. The p values were calculated with non-parametric Wilcoxon rank-sum tests. N, paired normal adjacent breast tissues; T, tumorous breast tissues. (D) Enzyme activity of HGPRT and 5′-NT in tumorous and paired normal adjacent tissues derived from patients with lung adenocarcinoma (n = 5). The p values were acquired by Student’s t test. (E) Differentially expressed PSP metabolites between tumorous and normal adjacent lung tissues from patients with lung cancer (n = 34). (F and G) Kaplan-Meier curves showing the association of expression of HPRT1 or NT5E and overall survival of patients with lung or breast cancer from the public TCGA and GTEx databases. The p values were gained by log-rank test. (H) A model proposed by the current study for the herbal compound OA. OA impaired cancer cell growth by blocking the PSP by degrading HGPRT and 5′-NT in this pathway through the SOD1/ROS/AMPK/mTORC1/autophagy/lysosome pathway.