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. 2021 Oct 11;11:20131. doi: 10.1038/s41598-021-99696-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of the interaction between rDIII and amplified phages using semi-quantitative ELISA. (A) Phage ELISA demonstrating the interaction between rDIII and C7C-mer and 12-mer phages amplified from the last round of biopanning. (B) Phage ELISA demonstrating the interaction of individual C7C-mer and 12-mer phage clones to rDIII. Framed reagents were coated into microtiter wells. Data present mean of triplicates with ± S.D. Asterisks indicate statistical significance. A statistical significance difference (P < 0.01, two-tailed P-value) was calculated with paired t-test. Statistics was performed with statistics tool of GraphPad Prism v8.4.3. In Panel (A)—a: P = 0.0013; b: P < 0.0001. In Panel (B)—a: P < 0.0001; b: P = 0.0002; c: P = 0.0007; d: P < 0.0001; e: P = 0.0014; f: P = 0.0010; g: P < 0.0001; h: P < 0.0001; i: P = 0.037 (ns). A – Absorbance; CB – coating buffer; rDIII – recombinant domain III; ns – non-significant; PA – primary antibody, CC – phage clone carrying 7-mer cyclic peptide; LC – phage clone carrying 12-mer linear peptide.